My Healing CommunityIntegrative Oncology Field Guide

WFA Evidence by Cancer Type

A cancer-type map for withaferin A, including stronger settings such as breast and ovarian cancer and thinner exploratory areas.

This section maps where WFA evidence is most clinically meaningful.

The strongest settings are those with multiple mechanistic papers, in vivo support, or early human relevance. Thinner signals remain worth noting, but they do not carry the same translational weight.

Stronger settings

Breast cancer

Breast cancer is the best-developed disease area for WFA. It anchors the autophagy blockade work, the ER-α suppression work, the vimentin and anti-metastatic work, and a large part of the apoptosis literature.

Ovarian cancer

Ovarian cancer is the most clinically advanced translational setting. The cisplatin combination work is strong, CSC biology is directly relevant, and an ongoing human trial exists.

Emerging settings

Non-small cell lung cancer

NSCLC stands out for EMT suppression, paclitaxel synergy, and checkpoint inhibitor sensitisation.

Glioblastoma

The biology is plausible because WFA targets HSP90, Akt/mTOR, and stem-like survival pathways. Clinical relevance remains limited by uncertainty around CNS delivery and relatively thin in vivo data.

Broader but thinner evidence base

WFA also has preclinical signals in colorectal, osteosarcoma, prostate, pancreatic, cervical, B-cell lymphoma, hepatocellular, and thyroid cancer.

These signals matter, but most do not yet justify the same level of confidence as breast or ovarian cancer.

Cancer-type overview

Cancer type

Key signal

Evidence level

Breast

Autophagy blockade, ER-α suppression, vimentin disruption, CSC relevance

Cell line and animal strong

Ovarian

Cisplatin synergy, CSC depletion, active trial landscape

Cell line, animal, and early human relevance

Non-small cell lung

EMT suppression, paclitaxel synergy, checkpoint sensitisation

Cell line and animal

Glioblastoma

HSP90/Akt/mTOR relevance, radiosensitisation, GSC interest

Thin but coherent

Colorectal

HIF-1α and EMT signalling

Cell line and animal

Osteosarcoma

Published Phase I oncology trial

Phase I human plus preclinical

Pancreatic

Oxaliplatin synergy and CSC relevance

Cell line only

Cervical

HPV oncoprotein relevance and xenograft activity

Cell line and animal

B-cell lymphoma

NF-κB, BCR signalling, and HSP90 disruption

Cell line and animal

Hepatocellular

Ferroptosis and resistance relevance

Cell line and animal

Thyroid

Sorafenib synergy

Cell line and animal

Explore by cancer type

Detailed pages in this section cover:

IMPORTANT: Please do not assume that any “ashwagandha” supplement will provide oncology‑relevant WFA exposure. This page was created to highlight Withaferin A‑focused targets, and specialised WFA‑standardised leaf extracts are required, not general ashwagandha root products. For support in sourcing see the Sourcing Quality page within this WFA in Oncology Hub.

Key references

Muniraj N. et al. — breast cancer autophagy and energetic impairment.
https://pmc.ncbi.nlm.nih.gov/articles/PMC10893887/

Sehdev V. et al. — ER-α suppression in ER-positive breast cancer.
https://pmc.ncbi.nlm.nih.gov/articles/PMC3129407/

Thaiparambil J. T. et al. — vimentin disruption and anti-metastatic activity in breast cancer.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0039065

Kakar S. et al. — ovarian cancer CSC targeting and cisplatin synergy.
https://pmc.ncbi.nlm.nih.gov/articles/PMC4218168/

Mukherjee A. et al. — ovarian cancer cachexia and muscle effects.
https://pmc.ncbi.nlm.nih.gov/articles/PMC12487364/

University of Louisville — ovarian cancer trial using Ashwagandha plus liposomal doxorubicin.
https://ctv.veeva.com/study/combination-therapy-for-recurrent-ovarian-cancer

Kim S. H. et al. — NSCLC EMT reversal and invasion.
https://onlinelibrary.wiley.com/doi/10.1002/mc.22110

Banerjee S. et al. — NSCLC checkpoint sensitisation.
https://pmc.ncbi.nlm.nih.gov/articles/PMC10295988/

Akbarshahi H. et al. — glioblastoma and brain-tumour models.
https://www.oncotarget.com/article/25175/text/

Bolleddula J. et al. — Osteosarcoma Phase I withaferin A trial.
https://www.sciencedirect.com/science/article/pii/S0975947618307897

Sun S. et al. — colorectal cancer growth and metastasis.
https://www.archivesofmedicalscience.com/Withaferin-A-inhibits-colorectal-cancer-growth-and-metastasis-by-targeting,196381,0,2.html

Sarin N. et al. — pancreatic cancer and oxaliplatin synergy.
https://www.sciencedirect.com/science/article/pii/S0753332218330701

Gopalakrishnan R. et al. — B-cell lymphoma.
https://uknowledge.uky.edu/microbio_facpub/159/

Guo J. et al. — hepatocellular carcinoma and ferroptosis review context.
https://onlinelibrary.wiley.com/doi/10.1002/ptr.8090

Access and availability:
Source: MCS Formulas, “Withaferin A Pro Liposomal.”
50 mg WFA per capsule. Available via healthcare professional request.
https://www.mcsformulas.com/vitamins-supplements/withaferin-a-pro-liposomal/ref/14

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